WHY IMUPRO Your building blocks for better health GOLD STANDARD OF IgG TESTING FOR DELAYED FOOD ALLERGIES

The German manufacturer R-Biopharm AG is leader in Clinical diagnostics and Food & feed analysis

 More than 15 years of experience in IgG testing

 R-Biopharm AG is ISO certified (ISO 9001 and ISO 13485)

 Kits are CE marked

 Standard curve calibrated against the international reference preparation “1st WHO IRP 67/86 for human IgG”

 Yearly interlaboratory tests

 Excellent reproducibility of the test results

 Standardized production of allergens

 Close cooperation with international scientific experts from nine countries

SOPHISTICATED DOCUMENTS FOR THE PATIENT

TO FACILITATE THE THERAPIST’S DAILY WORK!

 Detailed report

 Personal nutritional guidelines

 Individualized recipes (ImuPro Complete)

CONVINCING CONCEPT FOR SATISFIED PATIENTS

AND MEDICAL SUCCESS

 Improved concept developed on the basis of hundreds of thousands of patients’ cases and doctors’ experiences

(3 phases: elimination, provocation, rotation)

 Helps the patient to find his personal trigger foods

 Patient documents are highly personalized

 The ImuPro concept comprises the key to avoid malnutrition: rotation of all tolerated foods

 guaranteed varied diet supplying all necessary micro-nutriments and avoiding monotony

 helps to avoid unintended nutritional mistakes

(the guidelines point out in detail which products might be hidden in which processed foods)

 helps to avoid the appearance of IgG to newly introduced food

WHY CHOOSE IMUPRO?

IT WORKS • IT´S ACCURATE • IT´S INDIVIDUAL

WHY IgG INSTEAD OF

IgA OR IgG4?

Some laboratories and manufacturers use IgA to detect a delayed food allergy. Does this make sense?

Which antibody is best to detect a delayed food allergy?

OVERVIEW OF THE FUNCTIONS OF IgA, IgG4 AND IgG

IgA

 Principal isotype in secretions (esp. mucus epithelium of the intestinal and respiratory tracts)

 Neutralizing antibody against bacteria and toxins “first line of defense” with short half-life time (~6 days)

 Weak activator of complement and opsonisation – bound antigens are removed by macrophages with little to no inflammation

 Operates mainly on epithelial surfaces where complement and phagocytes are normally not present

 Is believed to act as a „discrete housekeeper“, in which foreign antigens are bound by IgA into complexes and removed by the

macrophages, but with little or no resultant inflammation

 Less specific antigen binding site than IgG, e.g. higher cross reactivity and more false positives

IgG4

 Antibody involved in the desensotisation of type I allergies (IgE)

 The guideline of the EAACI (European Academy of Allergy and Immunology) states that testing for IgG4 is not

recommended for the detection of delayed food allergies (https://www.ncbi.nlm.nih.gov/pubmed/18489614).

 Biochemically difficult to measure due to very low IgG4 concentrations and specificity of detection antibodies against IgG4

 Indicates tolerance not hypersensitivity

 Is not able to induce inflammation

 Is an anti-inflammatory antibody triggered by IL-10 (an anti-inflammatory cytokine)

IgG

 Principal isotype in the blood and extracellular fluid, where accessory cells and molecules are available

 Efficiently opsonises pathogens for engulfment by phagocytes and activates the complement system

 Persistent antibody with a half-life time of 20 days

 Induces inflammation

Vojdani* found a parallel in development of IgG and IgA antibodies to food, although less elevated levels

for IgA and in some cases no IgA response could be seen. In none of the cases presented, IgA was

positive alone; thus there seems to be no necessity to test for IgA alone.

* Detection of IgE, IgG, IgA and IgM antibodies against raw and processed food antigens.

Aristo Vojdani Nutrition & Metabolism 2009, 6:22

WHY CHOOSE IMUPRO?

• THERE IS NO ADDITIONAL BENEFIT IN MEASURING IgA TOO!

• MEASURING IgG4 IS NOT A RELEVANT MARKER FOR DELAYED FOOD ALLERGIES!

• IgG IS THE RELEVANT PARAMETER FOR IDENTIFYING POTENTIAL DELAYED FOOD

ALLERGIES IN THE HUMAN BLOOD!

IMUPRO AND COMPETITORS

Your building blocks for better health

Why ImuPro is the gold standard of IgG testing for delayed food allergies

TECHNICAL INFORMATION

ImuPro

 ELISA (Enzyme-linked immunosorbent assay) as a commonly accepted, accurate lab detection method

 Reliable and reproducible test results due to standardized procedures and simple pre analytical procedures.

Robust sample matrix. No cell based assays, which might be subject to difficult pre-analytical procedures

 Extensive technical validation (related to CLSI guidelines) of the single parameters (foods)

 Detection of the relevant biological marker (IgG)

 Standardized calibration curve according to 1st WHO IRP 67/86 for human IgG

 Calibration curve on each plate to avoid plate-to-plate variations

 Highly standardized and certified allergen production

 Pool test of food antigens avoided, to ensure most reliable information for each food stuff

 Quality control through annual Inter laboratory comparisons

Competitors:

 Various detection methods. Some are not generally accepted such as e.g. Bioresonance Therapy or Hair Testing

 Some competitors use complex and potentially less reliable test principles, such as cell based assays.

 To our knowledge, no or very basic technical validation data

 Some competitors claim to measure IgG4 or IgA

 Calibration curve according to 1st WHO IRP 67/86 for human IgG : Unclear / not standardized

 Calibration curve: several competitors either use only one curve or only use a one point calibration.

 Standardization of allergen production: No / not known

 Competitors often use pool tests to maximize the number of tested foods

 Interlaboratory tests: To our knowledge, the competitors do not conduct such tests

CONCEPT AND PATIENT CARE

ImuPro

 Comprehensive post-test guidance for patients

 Provocation challenge test as integrational part of the ImuPro concept

 Provocation enables the accurate identification of actual trigger foods

 Stabilization phase helps the patients to permanently improve their condition

 Several clinical trials conducted with ImuPro

 Comprehensive scientific support and advice

Competitors:

 No defined provocation concept

 No provocation challenge test

 No definite identification of trigger foods

 Stabilisation phase is not part of their concepts

 Very few publications

 If support at all, then very